|
Cellular kinetic 24 hs and 48 hs.
Once the optimum formulation and dosage
were determined according to inhibitory action of
growth up in BW cellular lines in 24 hours, it came
the experimentation the kinetic in 48 hours.
In TABLE 1: "Growth up' inhibition of BW cellular
line in 24 hours." They're presented the results
of the 2 formulations (PF1 y PF3), as a percentage
of growth up's inhibition of BW cellular lines in
24 hours, that in first phase of experimentation showed
significant differences in reference to inhibition
percentages of cellular growth up.
|
Dilution
|
PF1
|
PF3
|
|
Stimulation Rate
(%)
|
Inhibition (%)
|
Stimulation Rate
(%)
|
Inhibition
(%)
|
|
1/10
|
0.15
|
84.7
|
0.14
|
85.3
|
|
1/25
|
0.28
|
71.4
|
0.26
|
73.6
|
|
1/50
|
0.52
|
47.9
|
0.53
|
47.0
|
|
1/100
|
0.76
|
23.7
|
0.58
|
41.8
|
|
1/200
|
0.89
|
10.9
|
0.68
|
31.7
|
|
1/400
|
0.95
|
4.3
|
0.77
|
22.9
|
TABLE 1: Growth up's
inhibition in BW cellular line in 24 hs.
Again as we see in TABLE 1: "Inhibition of BW
cellular line's growth up in 24hs.", to bigger
dilutions the following percentages of inhibition
were decrecent, resulting the main efficacy with PF3
dilution.
In TABLE 2: "Inhibition of BW cellular line's
growth up in 48 hs.", the results are expressed
in percentages of inhibition of growth up in BW cellular
lines in 48 hours. Once more were included PF1 and
PF3 formulations as these one in the first phase of
experimentation showed relevant differences in reference
to the percentages of inhibition of cellular's growth
up, trying to discharge the possibility of a different
conduct at the 48 hours.
|
Dilution
|
PF1
|
PF3
|
|
Stimulation's Rate
(%)
|
Inhibition (%)
|
Stimulation's Rate
(%)
|
Inhibition
(%)
|
|
1/10
|
0.001
|
99.8
|
0.001
|
99.87
|
|
1/25
|
0.007
|
99.2
|
0.002
|
99.7
|
|
1/50
|
0.16
|
84.0
|
0.028
|
97.0
|
|
1/100
|
0.68
|
31.57
|
0.24
|
75.0
|
|
1/200
|
0.87
|
13.0
|
0.65
|
31.0
|
|
1/400
|
0.92
|
7.8
|
0.72
|
28.2
|
TABLE
2: Inhibition of BW cellular line's growth up in 48hs.
As a first conclusion in both formulations after 48
hours the inhibition effect at therapeutic dose was
bigger than the one in 24 hours, that shows an accumulation
effect (accumulation kinetic) to improve the wished
objective that is an non proliferation effect. Also
at 48 hours, the bigger inhibition of BW cellular
line's growth up in absolutes terms at the therapeutic
dose resulted in PF3 formulation
This way cellular kinetic is braked avoiding tumor's
growth up.
|
